abstract：:Oleaginous yeast Trichosporon cutaneum demonstrated excellent lipid accumulation performance and inhibitor tolerance derived from lignocellulose pretreatment. Here we firstly report a 30.45Mb assembly genome of T. cutaneum ACCC 20271 for understanding the microbial lipid biosynthesis and mechanism of inhibitor toleran...

journal_title：Journal of biotechnology

authors： Wang J,Gao Q,Bao J

更新日期：2016-06-20 00:00:00

abstract：:Cupriavidus nantongensis X1 is a chlorpyrifos degrading bacterium, which was isolated from sludge collected at the drain outlet of a chlorpyrifos manufacture plant. It is the first time to report the complete genome sequence of C. nantongensis species, which has been reported as a novel species of Cupriavidus genus. I...

journal_title：Journal of biotechnology

authors： Fang LC,Chen YF,Zhou YL,Wang DS,Sun LN,Tang XY,Hua RM

更新日期：2016-06-10 00:00:00

abstract：:Thioalkalivibrio versutus D301 isolated from Soda Lake is a haloalkaliphilic and obligated chemolithoautotrophic Gram-negative bacterium. The strain has a good adaption to hyperhaline and highly alkaline environment and a powerful sulfur-oxidizing ability. Here, we present the complete genome sequence of T. versutus D...

journal_title：Journal of biotechnology

authors： Mu T,Zhou J,Yang M,Xing J

更新日期：2016-06-10 00:00:00

abstract：:The methylotropic yeast Pichia pastoris has demonstrated its suitability for large-scale production of recombinant proteins. As an eukaryotic organism P. pastoris presents a series of advantages at expression and processing of heterologous proteins when compared with Escherichia coli. In this work, P. pastoris has bee...

journal_title：Journal of biotechnology

authors： de la Cruz S,Alcocer M,Madrid R,García A,Martín R,González I,García T

更新日期：2016-06-10 00:00:00

abstract：:Pedobacter cryoconitis PAMC 27485, an aerobic, Gram-negative, facultatively psychrophilic bacterium, was isolated from Antarctic soil. Here we report the complete genome of P. cryoconitis PAMC 27485, which contains a type II CRISPR-Cas system and genes encoding useful enzymes (e.g. proteases). The genome sequence of P...

journal_title：Journal of biotechnology

authors： Lee J,Jung YJ,Lee HK,Hong SG,Kim OS

更新日期：2016-05-20 00:00:00

abstract：:As oleaginous microorganisms represent an upcoming novel feedstock for the biotechnological production of lipids or lipid-derived biofuels, we searched for novel, lipid-producing strains in desert soil. This was encouraged by the hypothesis that neutral lipids represent an ideal storage compound, especially under arid...

journal_title：Journal of biotechnology

authors： Röttig A,Hauschild P,Madkour MH,Al-Ansari AM,Almakishah NH,Steinbüchel A

更新日期：2016-05-10 00:00:00

abstract：:Preussia sp. BSL10, family Sporormiaceae, was actively producing phytohormone (indole-3-acetic acid) and extra-cellular enzymes (phosphatases and glucosidases). The fungus was also promoting the growth of arid-land tree-Boswellia sacra. Looking at such prospects of this fungus, we sequenced its draft genome for the fi...

journal_title：Journal of biotechnology

authors： Khan AL,Asaf S,Khan AR,Al-Harrasi A,Al-Rawahi A,Lee IJ

更新日期：2016-05-10 00:00:00

abstract：:Glycosyltransferase (GT)-mediated methodology is recognized as one of the most practical approaches for large-scale production of glycosides. However, GT enzymes require a sugar nucleotide as donor substrate that must be generated in situ for preparative applications by recycling of the nucleotide moiety, e.g. by sucr...

journal_title：Journal of biotechnology

authors： Huang FC,Hinkelmann J,Hermenau A,Schwab W

更新日期：2016-04-20 00:00:00

abstract：:Two cyanobacterial strains, Synechocystis sp. PCC 6803 and Nostoc punctiforme ATCC 29133 were immobilized within magnesium phosphate based cements, showing a viability and activity for at least 4 weeks. These biohybrids are considered as an alternative photobioreactor material for bioremediation or an improved yield o...

journal_title：Journal of biotechnology

authors： Vorndran E,Lindberg P

更新日期：2016-04-10 00:00:00

abstract：:Lentinula edodes, the popular shiitake mushroom, is one of the most important cultivated edible mushrooms. It is used as a food and for medicinal purposes. Here, we present the 46.1 Mb draft genome of L. edodes, comprising 13,028 predicted gene models. The genome assembly consists of 31 scaffolds. Gene annotation prov...

journal_title：Journal of biotechnology

authors： Shim D,Park SG,Kim K,Bae W,Lee GW,Ha BS,Ro HS,Kim M,Ryoo R,Rhee SK,Nou IS,Koo CD,Hong CP,Ryu H

更新日期：2016-04-10 00:00:00

abstract：:Streptomyces globisporus C-1027 produces a nine-membered enediyne antitumor antibiotic lidamycin. Here we report the complete genome sequence of S. globisporus C-1027, which consists of a 7,608,611 bp linear chromosome, a 167,754 bp linear plasmid SGLP1 and a 7,234 bp circular plasmid pSGL1. The biosynthetic gene clus...

journal_title：Journal of biotechnology

authors： Li X,Lei X,Zhang C,Jiang Z,Shi Y,Wang S,Wang L,Hong B

更新日期：2016-03-20 00:00:00

abstract：:Extracts of the medicinal plant Hypericum perforatum are used to treat depression and skin irritation. A major API is hyperforin, characterized by sensitivity to light, oxygen and temperature. Total synthesis of hyperforin is challenging and its content in field-grown plants is variable. We have established in vitro c...

journal_title：Journal of biotechnology

authors： Gaid M,Haas P,Beuerle T,Scholl S,Beerhues L

更新日期：2016-03-20 00:00:00

abstract：:We previously showed that creation of a double strand DNA break (DSB) by expressing I-SceI in an engineered Trichoderma reesei (Hypocrea jecorina) strain containing a I-SceI recognition site improved transformation and homologous integration efficiencies. In this study, we further improved homologous integration frequ...

journal_title：Journal of biotechnology

authors： Ouedraogo JP,Arentshorst M,Nikolaev I,Barends S,Ram AF

更新日期：2016-03-20 00:00:00

abstract：:Kinetic models have a great potential for metabolic engineering applications. They can be used for testing which genetic and regulatory modifications can increase the production of metabolites of interest, while simultaneously monitoring other key functions of the host organism. This work presents a methodology for in...

journal_title：Journal of biotechnology

authors： Villaverde AF,Bongard S,Mauch K,Balsa-Canto E,Banga JR

更新日期：2016-03-20 00:00:00

abstract：:An NADH-dependent phenylpyruvate reductase (LaPPR) was identified through screening the shotgun library of Lactobacillus sp. CGMCC 9967. It belongs to D-3-phosphoglycerate dehydrogenase (PGDH) subfamily of 2-hydroxy acid dehydrogenase superfamily. LaPPR was stable at pH 6.5 and 30 °C, with a half-life of 152 h. LaPPR ...

journal_title：Journal of biotechnology

authors： Xu GC,Zhang LL,Ni Y

更新日期：2016-03-20 00:00:00

abstract：:Improving in vivo half-life and in vitro stability of protein-based therapeutics is a current challenge for the biopharmaceutical industry. In particular, recombinant human interferon alpha-2b (rhIFN-α2b), which belongs to a group of cytokines extensively used for the treatment of viral diseases and cancers, shows a p...

journal_title：Journal of biotechnology

authors： Ceaglio N,Gugliotta A,Tardivo MB,Cravero D,Etcheverrigaray M,Kratje R,Oggero M

更新日期：2016-03-10 00:00:00

abstract：:Planococcus rifietoensis M8(T) (=DSM 15069(T)=ATCC BAA-790(T)) is a halotolerant bacterium with potential plant growth promoting properties isolated from an algal mat collected from a sulfurous spring in Campania (Italy). This paper presents the first complete genome of P. rifietoensis M8(T). Genes coding for various ...

journal_title：Journal of biotechnology

authors： See-Too WS,Convey P,Pearce DA,Lim YL,Ee R,Yin WF,Chan KG

更新日期：2016-03-10 00:00:00

abstract：:Anaerobic co-digestion using protein-rich and lipid-rich co-substrates is limited by the accumulation of ammonia and long chain fatty acids (LCFAs), which are important inhibitors of the anaerobic microorganisms. This work aimed to study the microbial community dynamics during gradual and abrupt increase in ammonium a...

journal_title：Journal of biotechnology

authors： Regueiro L,Carballa M,Lema JM

更新日期：2016-02-20 00:00:00

abstract：:In this work, the ethanolysis of triglycerides catalyzed by immobilized lipase was studied, focusing on the secondary reaction of acyl migration. The catalytic tests were performed in a solvent-free reaction medium using Novozym 435 as biocatalyst. The selected experimental variables were biocatalyst loading (5-20mg),...

journal_title：Journal of biotechnology

authors： Sánchez DA,Tonetto GM,Ferreira ML

更新日期：2016-02-20 00:00:00

abstract：:The enzymatic bioconversion of chitin is of considerable interest for the natural production of bioactive compounds such as chitooligosaccharides and N-acetyl-d-glucosamine (GlcNAc). Key enzymes are involved in the natural processing of chitin, hydrolyzing this abundant biopolymer to yield chitooligosaccharides with s...

journal_title：Journal of biotechnology

authors： Nguyen-Thi N,Doucet N

更新日期：2016-02-20 00:00:00

abstract：:Roseateles depolymerans is a Gram-negative, rod-shaped, flagellated, obligately aerobic, photosynthetic bacterium that was isolated from the Hanamuro River, Ibaraki Prefecture, Japan. Here, we report the complete genome of R. depolymerans KCTC 42856(T) (=61A(T)=DSM 11813(T)=CCUG 48747(T)=NCIMB 13588(T)), which consist...

journal_title：Journal of biotechnology

authors： Lee SH,Choe H,Kim SG,Park DS,Nasir A,Kim BK,Kim KM

更新日期：2016-02-20 00:00:00

abstract：:Leuconostoc species are widespread in the natural environment and play an important role in several types of industrial and food fermentation processes. Here, we report the 1,854,727-bp complete genome sequence of the Leuconostoc mesenteroides ssp. dextranicum strain DSM 20484(T). ...

journal_title：Journal of biotechnology

authors： Park GS,Hong SJ,Jung BK,Lee C,Park CK,Shin JH

更新日期：2016-02-10 00:00:00

abstract：:Bacillus methylotrophicus JJ-D34 showing good proteolytic and antipathogenic activities was isolated from doenjang, a Korean traditional fermented soybean paste. Here, we report the complete genome sequence of strain JJ-D34 harboring a 4,105,955 bp circular chromosome encoding 4044 genes with a 46.24% G+C content, whi...

journal_title：Journal of biotechnology

authors： Jung JY,Chun BH,Moon JY,Yeo SH,Jeon CO

更新日期：2016-02-10 00:00:00

abstract：:In this study, kojic acid, a secondary metabolite as an industrially important compound, was produced by Aspergillus oryzae (A. oryzae), which was immobilized in plastic composite support (PCS) bioreactor. Nitrogen deficient medium was applied to increase the production of KA in PCS-immobilized bioreactor. The efficie...

journal_title：Journal of biotechnology

authors： Liu JM,Yu TC,Lin SP,Hsu RJ,Hsu KD,Cheng KC

更新日期：2016-01-20 00:00:00

abstract：:In our previous work, a NAD(H)-dependent carbonyl reductase (GoCR) was identified from Gluconobacter oxydans, which showed moderate to high enantiospecificity for the reduction of different kinds of prochiral ketones. In the present study, the crystal structure of GoCR was determined at 1.65Å resolution, and a computa...

journal_title：Journal of biotechnology

authors： Deng J,Yao Z,Chen K,Yuan YA,Lin J,Wei D

更新日期：2016-01-10 00:00:00

abstract：:Pandoraea thiooxydans DSM 25325(T) is a thiosulfate-oxidizing bacterium isolated from rhizosphere soils of a sesame plant. Here, we present the first complete genome of P. thiooxydans DSM 25325(T). Several genes involved in thiosulfate oxidation and biodegradation of aromatic compounds were identified. ...

journal_title：Journal of biotechnology

authors： Yong D,Ee R,Lim YL,Yu CY,Ang GY,How KY,Tee KK,Yin WF,Chan KG

更新日期：2016-01-10 00:00:00

abstract：:A Gram-negative, rod-shaped, non-motile, gamma and UV radiation resistant bacterium Hymenobacter radioresistens DG25B was isolated from a soil sample collected in South Korea. The complete genome sequence of H. radioresistens DG25B consists of one circular chromosome (3,874,646 bp). The bacterium was isolated from gam...

journal_title：Journal of biotechnology

authors： Kim MK,Joo ES,Lee SY,Lee DS,Srinivasan S,Jung HY

更新日期：2016-01-10 00:00:00

abstract：:To explore the utility of glycosyltransferases as novel biocatalysts, we isolated the glycosyltransferase genes CaUGT2 and SbUGTA1 from Catharanthus roseus and Starmerella bombicola, respectively and heterologously expressed them in Escherichia coli. The purified recombinant proteins were assayed with a variety of sma...

journal_title：Journal of biotechnology

authors： Huang FC,Hinkelmann J,Schwab W

更新日期：2015-12-20 00:00:00

abstract：:Rufibacter tibetensis strain 1351, isolated from the soil of the Tibet plateau of China, belongs to the family of Cytophagaceae. It is a red-pigmented, gram-negative, strictly aerobic and rod-shaped bacterium and shows resistance to UV radiation. Here, we report its complete genome sequence, which can help us find the...

journal_title：Journal of biotechnology

authors： Zhang Y,Yu C,Zhou M,Tang J,Li X,Wang Z,Li Z,Yao J,Li P,Zheng G,Chen X,Dai J

更新日期：2015-12-20 00:00:00

abstract：:A novel bacterial species was isolated from an industrial-scale biogas plant. The isolate Herbinix hemicellulosilytica T3/55(T) is able to degrade crystalline cellulose. Comparative 16S rRNA gene sequencing demonstrated that the isolate is closely related to environmental samples forming a hitherto unknown sub-cluster...

journal_title：Journal of biotechnology

authors： Koeck DE,Maus I,Wibberg D,Winkler A,Zverlov VV,Liebl W,Pühler A,Schwarz WH,Schlüter A

更新日期：2015-11-20 00:00:00

abstract：:Photorhabdus temperata subsp. thracensis 39-8(T), a symbiotic bacterium from an entomopathogenic nematode Heterorhabditis bacteriophora, is a novel bacterium harboring insect pathogenicity. Herein, we present the complete genome sequence of strain 39-8(T), which consists of one circular chromosome of 5,147,098 bp with...

journal_title：Journal of biotechnology

authors： Kwak Y,Shin JH

更新日期：2015-11-20 00:00:00

abstract：:The volatile fatty acid propionate inhibits anaerobic digestion during organic waste treatments. To examine potential microbial interactions that accelerate propionate oxidation, anaerobic digestion systems seeded with various types of anaerobic sludge were analyzed. Seed samples were collected from 10 different full-...

journal_title：Journal of biotechnology

authors： Kim W,Shin SG,Han G,Cho K,Hwang S

更新日期：2015-11-20 00:00:00

abstract：:Kinetic modelling, molecular modelling, and experimental determination of the initial reaction velocity of lipase-catalyzed alcoholysis were combined to study the effect of the alcohol substrate to catalytic activity. The model system consisted of methanol or ethanol at varying concentrations, vinyl acetate as ester s...

journal_title：Journal of biotechnology

authors： Sasso F,Kulschewski T,Secundo F,Lotti M,Pleiss J

更新日期：2015-11-20 00:00:00

abstract：:Streptomyces sp. strain CFMR 7, which naturally degrades rubber, was isolated from a rubber plantation. Whole genome sequencing and assembly resulted in 2 contigs with total genome size of 8.248 Mb. Two latex clearing protein (lcp) genes which are responsible for rubber degrading activities were identified. ...

journal_title：Journal of biotechnology

authors： Nanthini J,Chia KH,Thottathil GP,Taylor TD,Kondo S,Najimudin N,Baybayan P,Singh S,Sudesh K

更新日期：2015-11-20 00:00:00

abstract：:The development and optimization of a purification process of monoclonal antibodies based on two continuous chromatography steps for capture and intermediate purification are presented. The two chromatography steps were individually optimized using either batch chromatography or sequential multicolumn chromatography (...

journal_title：Journal of biotechnology

authors： Girard V,Hilbold NJ,Ng CK,Pegon L,Chahim W,Rousset F,Monchois V

更新日期：2015-11-10 00:00:00

abstract：:In the current study, a three-tiered mutagenesis strategy was employed to simultaneously improve the thermostability and activity of halohydrin dehalogenase from Agrobacterium radiobacter AD1 (HheC) by engineering the last ten amino acids (Met245∼Glu254) of its C-terminal region. Initially, truncated mutagenesis resul...

journal_title：Journal of biotechnology

authors： Wang X,Han S,Yang Z,Tang L

更新日期：2015-10-20 00:00:00

abstract：:The kinetic characterization of the photosynthetic activity in autotrophic microalgae plays a key role in the design of optimized photobioreactors. This paper presents a procedure to assess kinetic parameters of a three-state photosynthetic reaction centres model. Four kinetic parameters of the model were assessed by ...

journal_title：Journal of biotechnology

authors： Gargano I,Olivieri G,Spasiano D,Andreozzi R,Pollio A,Marotta R,D'Ambrosio N,Marzocchella A

更新日期：2015-10-20 00:00:00

abstract：:Acetogenic bacteria like Acetobacterium woodii represent an ancient group of anaerobic microorganisms which use hydrogen and carbon dioxide to produce acetate. Cell concentrations and space-time yields are usually low in gas fermentations. A standard stirred‑tank bioreactor with continuous gas supply was equipped with...

journal_title：Journal of biotechnology

authors： Kantzow C,Mayer A,Weuster-Botz D

更新日期：2015-10-20 00:00:00

abstract：:Fermented Monascus pigments have been utilized as traditional Chinese medicine and food colorant for thousands of years. Under the limited nitrogen concentration and/or low initial pH 2.5 conditions, it was observed that production of intracellular pigments and accumulation of microbial lipids (high content reaching t...

journal_title：Journal of biotechnology

authors： Wang B,Zhang X,Wu Z,Wang Z

更新日期：2015-10-20 00:00:00

abstract：:The detection of gene promoter methylation plays increasing roles in personalized medicine. In this study, an improved gene promoter methylation assay based on fluorescence polarization in 5'-nuclease reaction was developed. The novel assay offered a homogeneous annealing and cleavage reaction fully integrated with PC...

journal_title：Journal of biotechnology

authors： Su H,Wu Z,Wang Y,Jiang Y,Qiang S,Cheng H,Liu W,Zhang J

更新日期：2015-10-10 00:00:00